Cathepsin B of Schistosoma mansoni

Characterization of the cathepsin-like cysteine proteinases of Schistosoma mansoni.

Adult Schistosoma mansoni parasites synthesize and secrete both cathepsin L and cathepsin B cysteine proteinases. These cysteine proteinase activities, believed to be involved in hemoglobin digestion by adult schistosomes, were characterized by using specific fluorogenic peptide substrates and zymography. Both cathepsin L- and B-like activities with pH optima of 5.2 and 6.2, respectively, predo...

Biochemical characterization of cathepsin D from adult Schistosoma mansoni worms.

Functional expression and characterization of Schistosoma mansoni cathepsin B and its trans-activation by an endogenous asparaginyl endopeptidase.

Peptidases are essential for the establishment and survival of the medically important parasite, Schistosoma mansoni. This helminth expresses a number of gut-associated peptidases that degrade host blood proteins, including hemoglobin, as a means of nutrition. Using irreversible affinity probes, we demonstrate that S. mansoni cathepsin B-like endopeptidase 1 (SmCB1) is the most abundant papain ...

Carbohydrate Metabolism of Schistosoma Mansoni* B~ Ernest Bueding

In contrast to vertebrates and to bacteria relatively little is known about the metabolism of invertebrates (1). For a better understanding of comparative biochemistry information about this subject is required. Furthermore, investigations of the biochemical characteristics of parasitic invertebrates will reveal metabolic reactions essential for survival of these organisms. Information obtained...

Carbohydrate Metabolism of Schistosoma Mansoni* B~ Ernest Bueding

In contrast to vertebrates and to bacteria relatively little is known about the metabolism of invertebrates (1). For a better understanding of comparative biochemistry information about this subject is required. Furthermore, investigations of the biochemical characteristics of parasitic invertebrates will reveal metabolic reactions essential for survival of these organisms. Information obtained...